Restriction Enzymes
New England Biolabs, Inc. MfeI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence C^AATT_G. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. HaeIII – 3000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. AscI – 2500 units
A restriction endonuclease that recognizes the sequence GG^CGCG_CC.
New England Biolabs, Inc. NaeI – 500 units
A restriction endonuclease that recognizes the sequence GCC_^GGC.
New England Biolabs, Inc. SphI-HF – 500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. KpnI-HF – 20000 units
A high fidelity restriction endonuclease that recognizes the sequence G_GTAC^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. NheI-HF – 5000 units
A high fidelity restriction endonuclease that recognizes the sequence G^CTAGC. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. BbsI HF - 1000 units
A high fidelity restriction endonuclease that recognizes the sequence GAAGAC(N)2. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind HF restriction enzymes are fully active under a broader range of conditions minimizing off-target products while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. XmnI – 5000 units
A restriction endonuclease that recognizes the sequence GAANN_^NNTTC.
New England Biolabs, Inc. SfoI – 500 units
A restriction endonuclease that recognizes the sequence GGC_^GCC.
New England Biolabs, Inc. NdeI – 4000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. XhoI – 5000 units
A restriction endonuclease that recognizes the sequence C^TCGA_G.
New England Biolabs, Inc. BmtI-HF – 300 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. BsaI-HF®v2 – 5000 units
BsaI-HFv2 is a Type IIS restriction enzyme that has been optimized for Golden Gate Assembly and in protocols requiring DNA cutting by BsaI. Works well for any protocol requiring DNA cutting by BsaI. Recommended enzyme for any purpose requiring digestion at the recognition sequence ...5-GGTCTC(N1)/(N5)-3...
- Engineered for improved performance
- Used in Golden Gate Assembly; maintains high activity levels in T4 DNA Ligase Buffer
- Type IIS REs recognize asymmetric DNA sequences; cleave outside their recognition sequence
- 100% activity in rCutSmart Buffer
- Time-Saver qualified for digestion in 5-15 minutes
